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. 2008 Nov;148(3):1497–1509. doi: 10.1104/pp.108.123356

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Copyright © 2008, American Society of Plant Biologists

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Figure 2. — Generation of XA27-tagged lines. A, Disease phenotypes of XA27-tagged lines, control lines, and wild-type plants at 14 DAI with X. oryzae pv oryzae strains. Plants were inoculated with X. oryzae pv oryzae PXO99^A harboring wild-type avrXa27 or avrXa27 mutant PXO99^AME1 (ME1), and photographs were taken at 14 DAI. IRBB27, wild-type Xa27 plants; Ni, Nipponbare; GFP, L8 of P_Ubi:GFP:T_Nos; XA27-GFP(L22), L22 of P_Xa27:XA27-GFP:T_Xa27; XA27-GFP(L9), L9 of P_Ubi:XA27-GFP:T_Nos; XA27G-GFP, L18G of P_Ubi:XA27G-GFP:T_Nos; XA27-FLAG, L18F of P_Xa27:XA27-FLAG:T_Xa27. B, Expression of P_Xa27:XA27-GFP:T_Xa27 in uninoculated (UI) L22 plants or L22 plants at 3 DAI with X. oryzae pv oryzae strains PXO99^AME1(ME1) and PXO99^A. Nipponbare (Ni) was used as a control. C, Ubiquitin promoter-driven GFP, XA27-GFP, and XA27G-GFP gene expression in transgenic lines. GFP, L8 of P_Ubi:GFP:T_Nos; XA27-GFP(L9), L9 of P_Ubi:XA27-GFP:T_Nos; XA27G-GFP, L18G of P_Ubi:XA27G-GFP:T_Nos. Nipponbare (Ni) was used as a control. D, Expression of P_Xa27:XA27-FLAG:T_Xa27 in uninoculated (UI) L18F plants or L18F plants at 3 DAI with PXO99^AME1(ME1) or PXO99^A. Nipponbare (Ni) was used as a control. E, Expression of XA27-FLAG in uninoculated (UI) L18F plants or L18F plants at 3 DAI with PXO99^AME1(ME1) or PXO99^A. Nipponbare (Ni) was used as a control. XA27-FLAG was detected with anti-FLAG monoclonal antibodies. The molecular mass values of standard protein markers (Amersham Biosciences; RPN755) are shown in kilodaltons. The position of XA27-FLAG is indicated (arrowhead).