Skip to main content
. Author manuscript; available in PMC: 2009 Sep 26.
Published in final edited form as: Mol Cell. 2008 Sep 26;31(6):909–917. doi: 10.1016/j.molcel.2008.08.027

Figure 1.

Figure 1

Uch37 associates with both proteasome and hINO80. (A) Affinity-purified 2XHA-Uch37 and its co-purified proteins from cytoplasm (S100) or the nuclei (NE) of HEK293 cells were analyzed by SDS-PAGE and silver staining. Bovine 26S proteasome (0.5 μg) (Hoffman et al., 1992) and hINO80 affinity-purified through Flag-CCDC95 were included for comparison. To facilitate recovery of 26S proteasomes, 3 mM ATPγS was included in buffers during isolation of Uch37-containing complexes in lanes 2 and 3. (B) The samples shown in (A) were analyzed by mass spectrometry. Numbers are NSAFs (Normalized Spectral Count Abundance Factors) (Zybailov et al., 2006) multiplied by 1000. Proteins listed were not detected in mock purifications from parental HEK293 cells.