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. Author manuscript; available in PMC: 2009 Sep 15.
Published in final edited form as: J Immunol. 2008 Sep 15;181(6):4279–4286. doi: 10.4049/jimmunol.181.6.4279

Figure 2. Chitin regulation of macrophage IL-17AR.

Figure 2

Peritoneal macrophages were incubated with chitin (40-70μm; 100μg/ml) or vehicle control for 8h or other times when indicated. The effects of these treatments on IL-17R mRNA (A) and IL-17R protein expression (B) were evaluated. IL-17R mRNA was quantified using real-time RT-PCR and is expressed as a % of the control (Ct) values. Macrophage intracellular IL-17R protein was assessed using FACS analysis. The upper panel compares the expression of the IL-17R in cells incubated in medium alone (dark) or medium plus chitin (line) (mean fluorescent intensity; MFI). The staining with the isotype control is shown in light gray. The lower panel illustrates the MFI values (mean ± SEM) quantitating the IL-17R protein expression on cells incubated in medium alone (hatched bars) and medium plus chitin (solid bars). * p < 0.05, ** p < 0.01, *** p < 0.001.