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. Author manuscript; available in PMC: 2009 Sep 15.
Published in final edited form as: J Immunol. 2008 Sep 15;181(6):4279–4286. doi: 10.4049/jimmunol.181.6.4279

Figure 4. Roles of TLR(s), MyD88 and IL-17 in chitin-induced cytokine and chemokine production.

Figure 4

Peritoneal macrophages were obtained from TLR, MyD88 and IL-17A sufficient (+/+; +) and deficient (−/−; −) mice. These cells were incubated with chitin (solid bars, 100 μg/ml) or vehicle control (hatched bars) for 8h. The effects of these treatments on IL-12/IL-23p40 (A), IL-23 (B), TNF (C), RANTES/CCL5 (D) and MIP-2/CXCL2 (E) protein production were assessed by ELISA. In panel F, cells were obtained from wild type and IL-17 null mice and incubated with rmIL-17 (100 ng/ml) or vehicle. They were then treated with chitin or vehicle for 8 hours and the levels of supernatant IL-23 were assessed by ELISA. ** p < 0.01, *** p < 0.001.