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. Author manuscript; available in PMC: 2009 Sep 15.
Published in final edited form as: J Immunol. 2008 Sep 15;181(6):4279–4286. doi: 10.4049/jimmunol.181.6.4279

Figure 6. Roles of TLR(s), MyD88 and IL-17 in chitin-induced cytokine production in vivo.

Figure 6

TLR, MyD88 and IL-17 sufficient (+/+; +) and deficient (−/−; −) as well as Rag2/γC null mice (B) mice were treated with intranasal chitin (solid bars or dot plots) or vehicle controls (hatched bars or dot plots). Six hours later the animals were sacrificed and BAL was undertaken. The effects of these treatments on intracellular IL-17 protein (A and B), IL-17AR protein (C), BAL IL-12/IL-23p40 protein accumulation (D) and BAL IL-23 protein accumulation (E) were evaluated. IL-12/IL-23p40 and IL-23 were assessed by ELISA. IL-17A and IL-17AR expression were assessed using FACS analysis. These results are shown as dot plots or expressed in MFI values (mean ± SEM) comparing the IL-17A or IL-17AR protein on cells incubated with medium alone (hatched bars) and medium plus chitin (solid bars). * p < 0.05, ** p < 0.01, *** p < 0.001.