Table 1.
| Table 1a. Matings with control and Arsenophonus-infected females to wild type males | |||||||
|---|---|---|---|---|---|---|---|
| Class (Female) | Nm | Daughters | (SE) | Sons | (SE) | %Sons | P |
| AsymC | 25 | 32.8 | (2.7) | 19.2 | (2.0) | 36.9 | |
| AsymC(INF) | 17 | 27.9 | (4.2) | 5.4 | (0.9) | 16.2 | <0.0014 |
| LabII | 25 | 29.3 | (2.4) | 14.6 | (2.3) | 33.3 | |
| LabII(INF) | 11 | 18.5 | (3.5) | 3.9 | (1.7) | 17.4 | <0.035 |
| Table 1b. Number of eggs laid and number of surviving pupae for virgin Arsenophonus-infected and control virgin uninfected females. Virgin females produce only haploid male offspring. | ||||||||
|---|---|---|---|---|---|---|---|---|
| Eggs | Pupae | |||||||
| Class | Nv | Ne | (SE) | Nv | Np | (SE) | %Survival | P |
| LabII | 47 | 26.06 | (1.44) | 45 | 22.91 | (1.26) | 87.9 | |
| LabII(INF) | 61 | 34.05 | (1.61) | 60 | 8.35 | (0.94) | 24.5 | <0.00001 |
Arsenophonus causes haploid male-specific lethality. (INF) denotes lines that are infected with Arsenophonus. LabII host lines, but not AsymC lines, are infected with Wolbachia. Nm is the number of mated females from each cross. SE is the standard error of the mean given for the number of daughters and the number of sons per mated female. The percent (%) sons for AsymC and LabII are below 50% due to reduced fertilization rates by mothers, but these values fall within the range of male progeny produced from Arsenophonus-uninfected females in previous studies. P values are shown for Mann-Whitney U tests comparing the % sons between each pair of laboratory line crosses.
Shown is the number of virgin females (Nv) provided with hosts, the average number of eggs laid per female (Ne) and the number of offspring surviving to the pupal stage (Np) from LabII (control) and LabII(INF) (Arsenophonus-infected) host lines. The standard error of the mean (SE) is shown for Ne and Np. %Survival is the ratio of eggs laid to surviving pupae. The P value is shown for bootstrap sampling with 10,000 replicates to test the difference in survival between the two classes. Note that the egg and pupal counts were made from different host sets, because opening the host to count eggs prevents their development to pupae.