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Expression of recA mRNA in BK4012 (tisAB::Kanr), AB1157 and AB1157 transformed with pBK410 (tisAB overexpression). Total RNA was extracted from the strains and 5 µg RNA obtained from unexposed cells (–) and cells exposed to UV (50 J/m2) at two different time points (5 and 20 min after UV-exposure) was separated on formaldehyde-1% agarose gel and blotted onto a nylon membrane. The blot was hybridized with a recA riboprobe.
