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. 2008 Oct 3;36(19):6309–6317. doi: 10.1093/nar/gkn615

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© 2008 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — 3AB does not affect in vitro telomerase activity. After 4 weeks of incubation of cells, in vitro telomerase activity of COM3 cell extracts were measured by conventional PCR-based assay (TRAP). (A) Representative gel with TRAP products; 3AB+ and 3AB− lanes were from the same gel and only rearranged to the control lane. 3AB+, continuously 3AB treated; 3AB−, 3 weeks after 3AB release; C, control cells; St, standard reaction for normalization; C RNAse, control sample treated with RNaseA; C Heat, control sample treated with heat; LB, lysis buffer only; St Heat, standard reaction treated with heat. (B) Evaluation of three independent experiments. The activity is expressed as total product generated (TPG) units. No significant differences were observed. Hatched bar, control; black bar, 4 mM 3AB; open bar, released.