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. 2008 Sep 27;36(19):6132–6142. doi: 10.1093/nar/gkn625

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© 2008 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Depletion of fission yeast Rps2 results in a deficit in 40S ribosomal subunits. Strain FBY136 was grown to early log-phase before being treated (C–F) or not treated (A and B) by the addition of thiamine to the culture media for 0 h (A and C), 4 h (D), 8 h (E) and 12 h (B and F). Cell extracts were prepared under low-Mg²⁺ ion concentration (40 mM EDTA) that causes all cellular ribosomes to dissociate into separate subunits. Eight A₂₅₄ units of each extract were resolved on 5–45% sucrose gradients and the absorbance (A₂₅₄) was continuously measured. The peaks corresponding to the 40S and 60S ribosomal subunits are indicated in (A and C).