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. 2008 Sep 10;36(19):e127. doi: 10.1093/nar/gkn566

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© 2008 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Acceptor vectors for endogenous epitope tagging. pAAV-SEPT-Acceptor (A) and pAAV-TK-Acceptor (B) both contain a FLOXed Neo^R gene flanked by polylinkers containing unique, rare-cutting restriction sites chosen to simplify four-way ligations. L-ITR and R-ITR refer to the inverted terminal repeats required for viral packaging. pAAV-SEPT-Acceptor contains a SEPT cassette composed of a splice acceptor (SA), internal ribosome entry site (IRES) and promoterless Neo^R gene. pAAV-TK-Acceptor contains a Neo^R gene driven by a thymidine kinase (TK) promoter.