Figure 2.

Gel mobility shift analysis of the interactions between PspGI and oligoduplexes containing A/T (A), G/C (B) and G/G (C) base pairs at the center of CC(N/N)GG site. PspGI binding to nonspecific NS oligoduplex, which lacks the recognition site, is shown in (D). The binding reactions contained ³³P-labeled oligoduplex (0.2 nM) and PspGI at concentrations indicated by each lane. Samples were analyzed by PAGE under nondenaturing conditions (see, Material and methods section).