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. 2008 Sep 27;36(19):6101–6108. doi: 10.1093/nar/gkn621

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© 2008 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Binding of oligoduplexes containing different base pairs at the center of CC(N/N)GG sequences by wt Ecl18kI (A), W61Y (B) and W61A (C) mutants of Ecl18kI, EcoRII-C (D) and PspGI (E). Abbreviations below the bars specify the central base pair in the oligoduplex, 2 stands for 2-AP, a dash (−) marks the abasic site; oligoduplex NS lacks the recognition sequence of Ecl18kI/PspGI/EcoRII-C. WC stands for the Watson–Crick base pair; sMM, for the symmetrical mismatch; aMM, for the asymmetric mismatch; AB, abasic sites. Apparent K_a values determined for different DNA oligoduplexes by gel shift assay (see, Materials and methods section) are plotted in the diagrams.