FIGURE 1.

Both RAGE and TG2 knockout mouse cartilage explants retain responsiveness to IL-1β. Mouse femoral head cartilage was isolated as described in Materials and Methods. IL-1β (10 ng/ml) was added and GAG release was measured at 48 h (A and B) and NO generation at 24 h (C and D) assayed using the Griess reaction. Data are representative of three separate experiments. *, p < 0.05 compared with unstimulated cells.