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. Author manuscript; available in PMC: 2009 Jun 1.
Published in final edited form as: Cancer Prev Res (Phila). 2008 Mar 31;1(1):45–55. doi: 10.1158/1940-6207.CAPR-08-0034

Fig. 3.

Fig. 3

ER-negative mammary tumor development and erbB2 expression. A, expression of erbB2 in the normal mammary tissue and in the mammary tumors of trigenic erbB2 mice. Normal mammary tissue and mammary tumors samples were collected and embedded in paraffin. The 4-μm slides were prepared for staining with anti-erbB2 antibody. ErbB2 staining was viewed under a standard microscope and pictures were taken at ×10 magnification. Representative pictures are shown. Bar, 100 μm. B, manual scoring of the intensity of erbB2 staining was done by a pathologist on double-blinded samples. Scoring scale: 0, no or negligible erbB2 staining; 1, moderate intensity of erbB2 staining; and 2, strong erbB2 staining. Average intensity scores for the normal mammary tissue and mammary tumors were plotted separately for comparison purpose. C, ERα immunohistochemical (IHC) staining was done and viewed under a standard microscope and pictures were taken at ×20 magnification. Representative pictures are shown. Bar, 50 μm.