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. 2008 Aug 18;28(20):6483–6495. doi: 10.1128/MCB.00288-08

FIG. 1.

FIG. 1.

EGFR1 and EGFRvIII, but not HER2, enhance TBP expression in human and mouse cell lines. (A) Receptor activation in cell lines stably expressing EGFR1 or EGFRvIII. Immunoblot analysis was performed with whole cell lysates (WCL) derived from serum-deprived U87 or NR6 stable cell lines treated as designated using phospho-EGFR1 or EGFR1 antibodies. (B) TBP expression is increased in cell lines stably expressing EGFR1 or EGFRvIII. Immunoblot analysis was performed with WCL as shown in panel A using TBP or β-actin antibodies. Protein levels were quantified by densitometry, and TBP levels were calculated by normalization to β-actin levels. The change was calculated relative to untreated parental control cell lines. (C) TBP expression is not regulated by HER2. Immunoblot analysis was performed with lysates derived from serum-deprived MCF7 parental and MCF7 stable cells overexpressing HER2 or the NR6 stable lines. Lysates were prepared and immunoprecipitated with HER2 antibody and subjected to immunoblot analysis with HER2 or phospho-tyrosine antibody. Direct immunoblot analysis was also performed using TBP and β-actin antibodies.