FIG. 7.

Myc epitope-tagging strategy for UOS3. (A) Graphical representation of the tagging strategy. To epitope tag UOS3, a quadruple Myc tag sequence (4×myc) was introduced into the b3D.DT∧H.∧D vector. 4×myc is followed by the 3′ untranslated region (3′UTR) of the Plasmodium berghei DHFR gene (∼1,000 bp). The region of UOS3 (∼1,400 bp) corresponding to the C terminus was cloned in frame (without the stop codon) with the 4×myc tag. The plasmid was linearized for parasite transfection at the BsaBI restriction site. (B) 4×myc tag integration analysis was performed on genomic DNA from the parental population of parasites transfected with the 4×myc plasmid (integration) and wild-type (wt) parasites (negative control) using primers as indicated in panel A.