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Identification of MKL1 phosphorylation sites. (A) Conserved sequence motifs found between aa 444 and 500 in the MKL/myocardin protein family. (B to E) HeLa cells expressing MKL1 and the indicated mutants were stimulated with serum for 30 min, lysed, and immunoblotted with anti-Flag antibody. The STS/A and STS/E mutants have changes of S449/T450/S454 to alanines and glutamates, respectively.
