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Phosphorylation of MKL1 at serine 454. (A and B) Serum-starved TO3T3 cells transiently transfected with MKL1 and the indicated mutants in the p3x-FLAG-CMV-7.1 vector (2 μg/35-mm dish) were treated with serum for 30 min and immunoblotted with antisera that recognize phospho-S454 or Flag-MKL1. (C) Serum-starved TO3T3 cells were incubated with serum for the times indicated and detected with anti-phospho-S454 and anti-MKL1 antibodies.
