FIG. 3.

Stable expression and functional assay of TER variants in the template region. (A) Northern blot of mature TER and a loading control (LC) detected in total RNA from cell extract. “Relative TER” indicates hybridization signal for TER after normalization to the LC and expressed relative to recombinant wild-type (WT) TER. (B) TRAP assay with 2.5-fold serial dilutions of extract. The internal control for PCR amplification is shown. (C) Telomeric restriction fragments detected with a telomeric repeat probe.