FIG. 5.
Affinity purification of telomerase RNPs by tagged TER. 293T cells were transfected to express combinations of untagged and/or tagged TER, with coexpression of FLAG3-tagged TERT in panel B as indicated. TERs were detected by Northern blotting along with a recovery control (RC) added to each sample before RNA extraction. Recombinant untagged TER was used as a marker on all blots and is shown in lane 1 of panel A. Relative loadings of input and bound (IP) samples were 5% and 100% in panel A or 2%, 10%, and 90% of sequential samples in panel B.