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. 2008 Aug 11;28(20):6208–6222. doi: 10.1128/MCB.00611-08

FIG. 8.

FIG. 8.

IRF-1 is not recruited to the IL-8 promoter following the exposure of carcinoma cells to CD40L. EJ cells were stimulated with CD40L for the indicated times prior to ChIP analysis as described in detail in Materials and Methods. One-tenth of the volume of the chromatin obtained was used for PCR as input, and the remaining volume was immunoprecipitated with anti-p65 (α-p65) or anti-IRF-1 antibody or subjected to mock (i.e., without antibody) precipitation. Precipitated DNA encompassing the IL-8 promoter was then assayed by PCR. Lane 1 corresponds to a DNA molecular size marker. −, absent; 15′, 15 min.