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SPC flow cytometry analysis protocol. Leukocytes in Matrigel with or without lactide were evaluated based on forward and side laser light scattering, and the circled populations were selected for further analysis. SPCs were enumerated based on the surface expression of CD34 and DNA staining using DRAQ5. CD34+ DRAQ5+ cells were then evaluated for the expression of CD31, Sca-1, CXCR4, CD133, and VEGFR2.
