FIG. 2.
Influence of CSIG levels on PTEN expression. 2BS cells were stably transfected with pIRES-CSIG (A) or pSilencer-CSIG (B) or the respective empty vectors. Whole-cell lysates were prepared and subjected to Western blot analysis to determine PTEN, p27Kip1, p16INK4a, and p21Cip1 levels, and β-actin was served as a loading Ctrl. Relative abundances of PTEN and p27Kip1 were measured by densitometry and are expressed as increases relative to their respective levels in empty-vector-transfected cells.