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Intracellular localization of OTT-MAL deletions in the absence of serum. NIH 3T3 cells were transfected with the HA-tagged OTT-MAL deletion mutant constructs indicated, and serum-starved cells were stained with anti-HA antibodies and phalloidin on the following day. Shown are immunofluorescence micrographs for OTT-MAL (green, upper panels) and merged images (lower panels). Nuclei were stained blue with Hoechst 33258.
