FIG. 6.

Ectopic expression of actin constructs is unable to regulate OTT-MAL. (A) Cells were cotransfected with MAL or OTT-MAL and either wild-type actin or nonpolymerizable actin R62D. SRF luciferase reporter activity was normalized to LacZ and is shown as n-fold activation compared to unstimulated control cells (un.). (B) Effects of actin constructs fused to an NLS on SRF activity. Increasing amounts (100 and 300 ng) of either wild-type NLS-actin (wt.) or NLS-actin R62D were cotransfected with vector control (mock), MAL, OTT-MAL, or OTT-MALΔRPEL into NIH 3T3 cells. One day after transfection, SRF-luciferase activity was determined and normalized to pRL-tk. Shown is the average of three independent experiments, with error bars indicating the SEM. w/o, without FCS.