FIG. 4.

MAZ transactivates the minimal MCK promoter. MM14 skeletal myocytes were transfected with constructs containing the CAT reporter under the control of the 80-bp MCK minimal promoter (−80MCKCAT), the MCK minimal promoter containing the M2-M3 mutation ([M2-M3]−80MCKCAT; sequence in Fig. 1A), or three copies of the wild-type MPEX site upstream of the MCK minimal promoter ([MPEX]₃−80MCKCAT). Each reporter construct was transfected with or without 0.5 μg of MAZ expression vector (MAZ) or pCGN control vector (CTL). Data are plotted as the mean value and standard deviation of relative CAT activity, with activity of −80MCKCAT set at 100.