Table 1 Primers and PCR‐RFLP conditions for polymorphism analysis of the HBB gene.

RefSNP ID	Primers (5′→3′)	Position	Variation	Tm	MgCl2	Restriction enzyme
	Up: ctt tgg gtt gta agt ga	−551,	C/T	60°C	2.5 mM	RsaI
	Down: ttg gga tat gta gat gg
Rs10768683	Up: tta ggc tgc tgg tgg tc	+16, intron2	C/G	58°C	2 mM	AvaII
	Down: caa tca ttc gtc tgt ttc c
	Up: tta ggc tgc tgg tgg tc	+340 relative to the TAA site, 3′‐UTR	A/T	56°C	2.5 mM	HinfI
	Down: caa tca ttc gtc tgt ttc c