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The effect of mutagenizing the St-Cbs on DNA-processing. Cells were injected with either pCE5 (lane 1) or pCE5mut (lane 2). Cells were allowed to finish macronuclear development and a PCR reaction was performed using the primer combination P9/P548 for specific detection of the modified precursor sequence. M, molecular weight marker (1 kb ladder, Gibco BRL).
