Table 1.
Primers used for construction of the deletion vectors and for PCR analysis
| Different deletion constructs | ||||||
| Primer | Sequence | pCE12 | pCE18 | pCE19 | pCE20 | α1-GFPtel- |
| P4 | 5'-GGATCCATCAGATAACTCGCAAC | 5' | ||||
| P548 | 5'-CTGCAGGTCGACTCTAGAGCTC | |||||
| P7 | 5'-CAGATACAACGTCCCTCAAC | |||||
| P8 | 5'-GCGGGATCCATCTTCATTTAAACTAGATG | 3' | 3' | 3' | ||
| P9 | 5'-GGCTCGAGTTGCTACTCTCAGATATTC | |||||
| P15 | 5'-CGGAGGTACCCCGATATTTAAAATCATTAATC | 3' | ||||
| P20 | 5'-CCGCAGGATCCTTGAGAGTCTGCCATTTAAC | 5' | ||||
| P23 | 5'-GTTAAATGGCAGACTCTCAAGAAGAAATGC | |||||
| P25 | 5'-CCGGATCCAGACTTTCAGAGCGATATCTAG | 5' | ||||
| P26 | 5'-GCGGATCCCTCTCAAAGTGTGACATATTCC | 5' | ||||
| P27 | 5'-CGCAGGATCCAGAACAGTGGATTCGGAGGGAAATC | 5' | ||||
| P28 | 5'-CCGAGGTACCGAGATAGAATCGATTAAATAATGGG | 3' | ||||
| pGFP2 | 5'-CGCGAATTCTCACTTGTACAGCTCCTCCATG | |||||
| pUCP1 | 5'-GTCGACCTGCAGGCATGCAAGCTT | |||||
| pUCP2 | 5'-CGGGTACCGAGCTCGAATTC | |||||
| pGEMP1 | 5'-CGCATGCTCCTCTAGACTCGAGGAATTCGG | |||||
| pGEMP2 | 5'-GCTATGCATCCAACGCGTTGGGAGCTCTCC | |||||
| PMUT2 | 5'-GGTCAGAAATACTAGTTGATTCAGATAAGAAAG | |||||
Sequences of the primers used for construction of the different deletion vectors and for PCR analysis after injection of these constructs into the developing macronucleus. The 5' and 3' primers used for construction of each deletion vector are indicated.