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. Author manuscript; available in PMC: 2008 Nov 3.
Published in final edited form as: Arch Biochem Biophys. 2006 Nov 2;457(1):16–28. doi: 10.1016/j.abb.2006.10.011

FIG. 9. GRP-75 phosphorylation in the cytoplasm does not cause its translocation to mitochondria.

FIG. 9

A, Serum starved cells were left untreated (control), or treated with Ang II (100 nM) for either 15 min or 30 min. Mitochondria and cytoplasmic fractions were isolated as described in the methods section. Mitochondrial proteins were solubilized and equal amounts of protein from cytoplasm and mitochondrial fraction were run on a 8% SDS-polyacrylamide gel, and immunoblotted with anti-GRP-75 antibody. B, the samples representing A was immunoblotted with phospho-specific anti-caveolin-1 antibody cross-reactive to phosphorylated GRP-75. These blots are representative of three independent experiments. CR= cross reactive.