Expression of KCNQ isoforms in A7r5 cells and adult rat aorta. Total RNA prepared from A7r5 cells (A7), adult rat thoracic aorta (Ao), or adult rat brain as a positive control (+) was reverse transcribed and subjected to PCR using primers specific for KCNQ1 through KCNQ5. Molecular weight marker (M) is a 100-bp ladder; 500 bp is indicated at left. Expected sizes of reaction products are KCNQ1, 453 bp; KCNQ2, 372 bp; KCNQ3, 424 bp; KCNQ4, 495 bp; and KCNQ5, 240 bp. Products were confirmed by DNA sequencing.