Table I.
Relative chromatographic peak areas for CS/DS samples partially digested with chondroitinase ABC. Chondroitinase ABC digestions were stopped at 30% completion, and separated using high performance SEC, as described in the Materials and Methods section. The relative peak areas for Δ-unsaturated dp2, dp4 and dp6 oligosaccharides are given.
| dp2 | dp4 | dp6 | >dp6 | |
|---|---|---|---|---|
| CSA | 48.35 | 9.16 | 9.98 | 32.5 |
| CSB | 38.92 | 18.27 | 18.53 | 24.3 |
| CSC | 55.30 | 19.19 | 9.48 | 16.0 |
| CS6 | 58.02 | 21.38 | 9.13 | 11.5 |
| DS18 | 35.80 | 14.48 | 16.59 | 33.1 |
| DS36 | 37.93 | 10.91 | 11.53 | 39.6 |
| DS50 | 43.13 | 15.24 | 12.71 | 28.9 |
| ACD | 39.80 | 15.72 | - | 44.5 |
| CD | 55.16 | 29.19 | - | 15.7 |
| SD | 42.69 | 23.03 | - | 34.3 |