Figure 5.
Membrane homogeneity n versus time at different peptide/lipid ratios and DC potentials. FSKRGY (top panel) and AGGKGF (bottom panel) was added at t = 10 min at the peptide/lipid ratios and DC bias potentials indicated. For all DC bias potentials and peptide concentrations, n decreased upon addition of FSKRGY. No such changes were observed upon addition of AGGKGF. In FSKRGY experiments, more positive membrane potentials resulted in more moderate changes; in addition, the changes were larger at higher peptide concentration. Value of n reached minimum within 1–2 minutes, followed by a partial relaxation with a time constant of about 8 min.