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. 2008 Oct 21;9:497. doi: 10.1186/1471-2164-9-497

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Copyright © 2008 Flibotte and Moerman; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Effects of varying some oligonucleotide constraints on resolving power. The negative (or absolute value) of the slope in the resolving power curve is shown for individual constraints on oligonucleotides when detecting one-copy deletions with 50-mer probes. The green (red) bars correspond to C. elegans (human) data. The individual oligonucleotide constraints that have been varied consist of (A) the self-folding energy, (B) the length of the longest homopolymer, (C) the 15-mer count, and (D) the melting temperature.