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Introduction of random mismatches in oligonucleotides of various lengths. LOESS regression of the difference in fluorescence intensity (in log2 scale) between the original and perturbed oligonucleotides as a function of the number of mismatches introduced in the oligonucleotide. Solid (dashed) lines correspond to C. elegans (human) data. Results are shown for oligonucleotides of length 50 (green lines), 60 (red lines) and 70 (blue lines).
