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. Author manuscript; available in PMC: 2009 Sep 30.
Published in final edited form as: Immunol Lett. 2008 Aug 13;120(1-2):108–116. doi: 10.1016/j.imlet.2008.07.010

Figure 1. pp28 MVA and US32 MVA vectors.

Figure 1

(A) Schematic representation of MVA transfer pMCO3 plasmid and recombinant MVA vector. The plasmid has a vaccinia synthetic promoter (Psyn) which drives the expression of pp28 or US32 gene, indicated as X gene, and the synthetic promoter P7.5 for the expression of the gus gene. pMCO3 plasmid targets MVA deletion region III via flanking (FL) regions 1 and 2. pp28 MVA and US32 MVA were generated via homologous recombination by transfecting pMCO3 plasmid into wild type MVA infected BHK-21 cells. gus bacterial marker gene was used for color selection of recombinant MVA. (B) Western blot detection of US32 and pp28 from corresponding recombinant MVA infected BHK-21 cells. Lane 1 and 3: respectively, cell lysates of US32 MVA and pp28 MVA infected BHK-21 cells. In lane 2 and 4: cell lysates of wild type MVA infected BHK-21 cells, as negative controls.