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. 2008 Oct 9;8:178. doi: 10.1186/1471-2180-8-178

Table 2.

Comparison of multiplex PCR to serotyping for identifying Salmonella O alleles B; C1; C2; D1 or E1; H1 alleles i; g,m; r or z10;and H2 alleles 1,2 or e,n,x

Phase 1 PCRs Phase 2 multiplex PCR
Antigenic Formula O muliplex PCR i/g,m multiplex PCR r/z10multiplex PCR
O H1 H2 S. enterica Serovars Animal Origin (n)1 B C1 C2 D1 E1 i g,m r z10 1,2 e,n,x
A a 1,5 Paratyphi A 1 (1) 0 0 0 1 0 0 0 0 0 1 0
B b 1,2 Paratyphi B 1 (1) 1 0 0 0 0 0 0 0 0 1 0
B e,h 1,2 Saintpaul 1(1) 1 0 0 0 0 0 0 0 0 1 0
B e,h 1,5 Reading 1(2) 2 0 0 0 0 0 0 0 0 1 0
B f,g - Derby 1(1) 1 0 0 0 0 0 1 0 0 0 0
B i 1,2 Typhimurium 1, 4–6 (74) 74 0 0 0 0 74 0 0 0 74 0
B l,v 1,7 Bredeney 1(1) 1 0 0 0 0 0 0 0 0 1 0
B 1,v e,n,z15 Brandenburg 1(2) 2 0 0 0 0 0 0 0 0 0 2
B b - Java 6(1) 1 0 0 0 0 0 0 0 0 0 0
B e,h e,n,x Chester 1(2) 2 0 0 0 0 0 0 0 0 0 2
B f,g,s - Agona 1 (1) 1 0 0 0 0 0 1 0 0 0 0
B r 1,2 Heidelberg 1, 3–6(24) 24 0 0 0 0 0 0 24 0 24 0
B z 1,5 Kiambu 1(1) 1 0 0 0 0 0 0 0 0 1 0
B z 1,7 Indiana 1(2) 2 0 0 0 0 0 0 0 0 2 0
B z10 1,2 Haifa 6 (1) 1 0 0 0 0 0 0 0 1 1 0
C1 b l,w Ohio 1(1) 0 1 0 0 0 0 0 0 0 0 0
C1 c 1,5 Choleraesuis 1, 6(6) 0 6 0 0 0 0 0 0 0 6 0
C1 c 1,5 Paratyphi C 1 (1) 0 1 0 0 0 0 0 0 0 1 0
C1 d l,w Livingstone 6(1) 0 1 0 0 0 0 0 0 0 0 0
C1 g,m,s - Montevideo 1, 5(12) 0 12 0 0 0 0 12 0 0 0 0
C1 k 1,5 Thompson 1(1) 0 1 0 0 0 0 0 0 0 1 0
C1 m,t - Oranienburg 1(1) 0 1 0 0 0 0 1 0 0 0 0
C1 z29 - Tennessee 1(1) 0 1 0 0 0 0 0 0 0 0 0
C1 e,h e,n,z15 Braenderup 1(2) 0 2 0 0 0 0 0 0 0 0 2
C1 r 1,5 Infantis 1(2) 0 2 0 0 0 0 0 2 0 2 0
C1 z10 e,n,z15 Mbandaka 1(14) 0 14 0 0 0 0 0 0 14 0 14
C1 z28 - Lille 1(1) 0 1 0 0 0 0 0 0 0 0 0
C2 d 1,2 Muenchen 5(3) 0 0 3 0 0 0 0 0 0 3 0
C2 e,h 1,2 Newport 4,5(1) 0 0 1 0 0 0 0 0 0 1 0
C2 i z6 Kentucky 1(24) 0 0 24 0 0 24 0 0 0 0 0
C2 z10 e,n,x Hadar 1 (10) 0 0 10 0 0 0 0 0 10 0 10
D1 a 1,5 Miami 5(1) 0 0 0 1 0 0 0 0 0 1 0
D1 a 1,5 Sendai 5(1) 0 0 0 1 0 0 0 0 0 1 0
D1 g,m - Enteritidis 1(20) 0 0 0 20 0 0 20 0 0 0 0
D1 g,p - Dublin 2, 6(3) 0 0 0 3 0 0 3 0 0 0 0
D1 l,v 1,5 Panama 1 (1) 0 0 0 1 0 0 0 0 0 1 0
D1 - - Gallinarum 1(4) 0 0 0 4 0 0 4 0 0 0 0
D1 f,g,t - Berta 1 (2) 0 0 0 2 0 0 2 0 0 0 0
D1 l,z28 1,5 Javiana 1 (1) 0 0 0 1 0 0 0 0 0 1 0
E1 e,h 1,5 Muenster 1(2) 0 0 0 0 2 0 0 0 0 2 0
E1 l,v 1,7 Give 1(2) 0 0 0 0 2 0 0 0 0 2 0
E1 e,h 1,6 Anatum 1, 5 (4) 0 0 0 0 4 0 0 0 0 4 0
E1 l,v 1,6 London 1(2) 0 0 0 0 2 0 0 0 0 2 0
Total 239 114 43 38 34 10 98 44 26 25 135 30
False Positives 0 0 0 1 0 0 24 0 0 30 18
False Negatives 0 0 0 0 0 0 0 0 0 0 0
Kappa2 1.0 1.0 1.0 0.98 1.0 1.0 0.58 1.0 1.0 0.75 0.54

11 = poultry; 2 = bovine; 3 = swine, 4 = other (includes dog, heron, horse, opossum, parrot, rabbit, and snake); 5 = human; and 6 = unknown. Numbers in parentheses indicate the numbers of isolates for each serovar.

2Agreement between PCR allelotyping and conventional serotyping results