Table 3.
Allelotyping PCR scheme for presumptive identification of S. enterica serovars Enteritidis, Hadar, Heidelberg, and Typhimurium
| O-multiplex | H1-multiplexes1 | H2-multiplex | Serovars | Sensitivity5 | Specificity5 |
| B | i | I2 | Typhimurium | 1.00 | 1.00 |
| B | r | I | Heidelberg | 1.00 | 1.00 |
| C2 | z10 | II3 | Hadar | 1.00 | 1.00 |
| A/D1 | g,m | -4 | Enteritidis | 1.00 | 0.96 |
1Identifies H1 alleles i; g,m; r; or z10
2 Covers H2 alleles 1,2; 1,5; 1,6; and 1,7
3 Covers H2 alleles e,n,x and e,n,z15
4 PCR negative for H2-multiplex
5Sensitivity and specificity of the allelotyping PCR scheme relative to conventional serotyping in identifying S. enterica serovars Enteritidis, Hadar, Heidelberg, and Typhimurium among the 239 isolates examined in this study.