Fig. 6.

Senescence markers in human GH-producing pituitary adenomas. (A) Immunohistochemistry of the same GH-secreting human adenoma sections stained for p21 (brown) and SA-β-gal activity (blue). (B) Confocal image of double fluorescence immunohistochemistry of p21 (green) and β-galactosidase (red) proteins coexpression in human pituitary adenomatous but not in normal adjacent tissue (Left). High resolution (×63) image of the same slide (Right). (C) Proposed model for p21-induced senescence in the hypoplastic Pttg-null pituitary gland and PTTG-overexpressing pituitary adenomas. Arrows depict proposed pathways.