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. Author manuscript; available in PMC: 2009 Oct 15.
Published in final edited form as: FEBS Lett. 2008 Sep 5;582(23-24):3353–3358. doi: 10.1016/j.febslet.2008.08.027

Fig. 3.

Fig. 3

Incorporation of serine and Cyc in a β-galactosidase/luciferase fusion reporter protein. Bars represent UAG read-through efficiency: M. barkeri tRNAPyl and either M. barkeri bacterial like SerRS (B-SerRS), M. barkeri methanogenic SerRSs (M-SerRS), E. coli SerRS (Ec-SerRS) or an empty expression vector (pET15 empty). As positive control, M. barkeri tRNAPyl and M. barkeri PylRS (Mb-PylRS) in the presence of Cyc was measured. Negative controls include measurements with either M-SerRS or B-SerRS without PylT.