Skip to main content
. Author manuscript; available in PMC: 2009 Oct 1.
Published in final edited form as: Protein Expr Purif. 2008 Jun 10;61(2):142–148. doi: 10.1016/j.pep.2008.05.013

Figure 6.

Figure 6

Bioluminescent activity of Metridia luciferase purified from E. coli (dashed line) and Sf9 insect (solid line) cells at equal protein concentrations of (5 ng/ml). The coelenterazine concentration was 2.4 µM in both experiments. The comparison was carried out in 0.3 M NaCl, 20 mM Tris–HCl pH 7.5, a condition optimizing the bioluminescence activity of Metridia luciferase purified from E. coli.