. Author manuscript; available in PMC: 2009 Oct 1.

Published in final edited form as: Protein Expr Purif. 2008 Jun 10;61(2):142–148. doi: 10.1016/j.pep.2008.05.013

Table 1.

Purification of Metridia luciferase

	Total protein (mg)	Total activity (RLU^† × 10⁶)	% activity recovered	Specific activity (RLU × 10⁶/mg)
	Culture media (sf9 cells)

Media	2300.0	27500.0	100	11.9
Precipitation with (NH₄)₂SO₄	1610.0	26125.0	95	16.2
His-Trap column	9.8	20993.8	75.6	2142.2
TEV digested, His-Trap column flow-through	5.4	12335.8	44.5	2284.4
HiTrap Q	4.2	9644.1	34.8	2296.0
Superdex 75	3.4	8261.3	29.3	2429.8

	Inclusion bodies (E. coli cells)

6 M urea extract of inclusion bodies	51.6	–	–	–
Sepharose Fast Flow (6 M urea)	7.4	–	–	–
Refolding	6.1	456.9	100	74.9
HiTrap Q	3.4	401.2	87.8	118
Superdex 75	0.6	332.3	72.8	553.8

^†

RLU – at maximum bioluminescent intensity