TABLE 3.
CLASSIFICATION OF 10 GENES IN THE CYSTIC FIBROSIS THERAPEUTIC GENE SIGNATURE
| Array*
|
PCR†
|
||||
|---|---|---|---|---|---|
| Gene | Description | Fold Change | P Values | Fold Change | P Value |
| Cell membrane molecules and receptors | |||||
| CD64 | Fcγ receptor IA | −2.4 | 0.004 | −2.1 | 0.007 |
| CD36 | Collagen type 1 receptor/thrombospondin receptor | −1.7 | <0.001 | −1.6 | <0.001 |
| CD163 | Hemoglobin scavenger receptor | −1.6 | 0.003 | −1.7 | 0.024 |
| TLR2 | Toll-like receptor 2 | −1.6 | 0.003 | −1.7 | 0.011 |
| HCA112 | Hepatocellular carcinoma-associated antigen 112 | −1.8 | 0.004 | −1.6 | 0.012 |
| PLXND1 | Plexin D1 | −1.7 | 0.005 | −1.3 | 0.083 |
| Immune response | |||||
| IL32 | Interleukin 32 | 1.4 | 0.033 | 1.4 | 0.001 |
| Matrix degradation/extravasation | |||||
| HPSE | Heparanase | −1.4 | 0.017 | −1.6 | 0.004 |
| ADAM9 | A disintegrin and metalloproteinase, meltrin gamma | −2.5 | 0.004 | −1.7 | 0.016 |
| CSPG2 | Versican, chondroitin sulfate proteoglycan 2 | −1.9 | <0.001 | −1.9 | 0.015 |
Definition of abbreviation: PCR = polymerase chain reaction.
Values are mean fold changes or P values calculated by paired t test after log transformation; n = 6 patient samples pre- and postantibiotic therapy from development cohort.
*
Pre- and postantibiotic therapy expression values from oligonucleotide arrays.
†
Pre- and postantibiotic therapy real-time PCR gene expression values as copies per 1,000 copies of housekeeping gene, hypoxanthine-phosphoribosyl transferase (HPRT).