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In vitro vascular tube formation by human microvascular endothelial cells type 1. (A) Short-fragment hyaluronan (sHA) induces vascular tube formation, an effect that is significantly enhanced by inter–α-trypsin inhibitor (IaI). Negative control was 0% fetal bovine serum (FBS). Positive control was 5% FBS. (B) Quantification of vascular tube formation (Dunnett's multiple comparison testing; n = 6 wells/group). Arrowheads indicate vascular nodes. Error bars represent SEM.
