Fig. 2.

Changes in circular dichroism induced by noncharged liposomes. Experimental conditions are detailed in Fig. 1. a CD spectroscopy between 220 and 320 nm was performed for 0.010 mg DNA mixed with 0 (black line), 0.2 (gray line), and 1.0 mM (light gray line) noncharged lipids (0.4, 0.6, and 0.8 mM lipid concentrations were excluded from the graph in order to clarify observations) and b changes in the 275-nm band as a function of lipid concentration. Data were expressed as mean of five experiments ± SD, and differences between groups were tested using the hypothesis test. Data denoted with an asterisk were significantly different with respect to DNA without liposomes (p < 0.05)