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HF determination in noncharged liposomes. Experimental conditions were the same as described in Fig. 3. The A570/A500 ratio (HF) was determined for liposomes (squares), liposomes mixed with DNA, 100:1 lipid/DNA mass ratio (circles), and DNA without liposomes (triangles). Lipid/MC540 molar ratio was 160:1, and lipid/DNA mass ratio was 100:1. Results are expressed as the mean of five experiments ± SD
