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. Author manuscript; available in PMC: 2008 Nov 4.
Published in final edited form as: Growth Horm IGF Res. 2007 Feb 5;17(2):113–121. doi: 10.1016/j.ghir.2006.12.004

Fig. 2. Representative IGFBP-2 immunohistochemistry in PPNAD.

Fig. 2

(A) PRKAR1A mutation-positive PPNAD section labeled with 1:50 rabbit anti-human IGFBP-2 antibody and visualized by the Envision Plus method using DAB color substrate and Harris’ haematoxylin counter-staining (original magnification X100).

(B) Adjacent section to that in Panel A, simultaneously processed the same way except using rabbit anti-human β amyloid precursor protein antibody as negative control primary antibody (original magnification X100).

(C) and (D) PRKAR1A mutation-negative PPNAD sections processed the same as (A) and (B), respectively (both original magnification X100).