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. Author manuscript; available in PMC: 2008 Nov 4.
Published in final edited form as: Exp Cell Res. 2008 May 29;314(14):2634–2642. doi: 10.1016/j.yexcr.2008.05.009

Figure 4.

Figure 4

Efficient induction of pluripotency genes depends on transcription and translation.

(A, B) Chromatin immunoprecipitation, using an RNA polymerase II antibody, on either untreated NCCIT or 293 cells (A) or 293T cells incubated in 46C ES cell extract for 0 to 6 hours (B). Chromatin immunoprecipitates were subjected to PCR using primers specific for GAPDH, Nanog (promoter 1), Oct4 and CRIPTO. W, water only control for PCR reaction. The Nanog promoter (C) Quantitative RT-PCR analysis after incubation of 293T cells for 6 hours in mouse 46C ES extract. The ES extract was optionally supplemented with 100 μM α-amanitin or 1 mg/ml cycloheximide at the start of the incubation. Expression is expressed relative to untreated 293T cells.