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. 2008 Nov 1;22(21):2926–2931. doi: 10.1101/gad.1677208

Figure 1.

Figure 1.

Tpr binds to Mad1 and Mad2 directly. (A,D) Total cell lysates were prepared from HeLa cells grown asynchronously or arrested in mitosis by nocodazole followed by mitotic shake-off to allow collection, and then subjected to immunoprecipitation analysis. (B,E) The indicated proteins were expressed in HEK293 cells and subjected to GST pull-down analysis. (C, lanes 1,2) Coomassie staining of purified recombinant GST-tagged proteins. (Lane 3–5) Autoradiography of in vitro translated Tpr proteins. (Lanes 6–11) GST pull-down analysis was performed, and the bound proteins were visualized by autoradiography. (F, lanes 1–4) Coomassie staining of purified recombinant GST-tagged proteins. (Lanes 5–8) GST pull-down analysis was performed, and the bound proteins were visualized by autoradiography.