Figure 3.

C/EBP response elements are found at the vast majority of PPARγ-binding regions. (A) Enrichment of C/EBP motifs. The PPARγ-binding locations were mined as in Figure 2, B and C. Shown is the logo of one C/EBP PWM among several that were enriched. (B) ChIP-QPCR analysis for C/EBPα and PPARγ at several novel PPARγ-binding regions that were computationally predicted to contain C/EBP response elements (see Supplemental Tables 1 and 5 for identification and location of the PPARγ-binding sites). An area of the insulin gene served as negative control for PPARγ and C/EBPα binding. Data are normalized to a site in the Arbp/36b4 gene and presented as mean ± SE, n = 3.